Co-production of Amylase and Protease by Locally Isolated Thermophilic Bacterium <i>Anoxybacillus rupiensis</i> T2 in Sterile and Non-sterile Media Using Waste Potato Peels as Substrate

Tuysuz E., Gonul-Baltaci N., Ömeroğlu M. A., Adıgüzel A., Taşkın M., Özkan H.

WASTE AND BIOMASS VALORIZATION, sa.12, ss.6793-6802, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s12649-020-00936-3
  • Dergi Adı: WASTE AND BIOMASS VALORIZATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, CAB Abstracts, Compendex, INSPEC, Veterinary Science Database
  • Sayfa Sayıları: ss.6793-6802
  • Anahtar Kelimeler: Anoxybacillus rupiensis T2, Thermophilic bacteria, Potato peel, Protease, Amylase, Co-production, SOLID-STATE FERMENTATION, ALPHA-AMYLASE, ALKALINE PROTEASE, LIPID PRODUCTION, ACID, 1,3-PROPANEDIOL, PURIFICATION, RESIDUES, MOLASSES, ENZYMES
  • Atatürk Üniversitesi Adresli: Evet

Özet

The present study investigated the potential of thermophilic bacteria isolated from hot springs to simultaneously produce protease and amylase enzymes. Among ten isolates, the strain T2 was found to be more favorable for amylase and protease. This strain was identified as Anoxybacillus rupiensis (GenBank number: MN252572). Potato peel powder (PPP) was used as a substrate for co-production of amylase and protease from A. rupiensis T2. Experiments were performed under sterile and non-sterile culture conditions. The optimal parameters for co-production of these enzymes were a PPP concentration of 60 g/L, temperature of 50 degrees C, initial pH of 7.0 and incubation time of 48 h. Under these culture conditions, the amylase and protease activities were determined as 64.9 and 26.2 U/mL in sterile medium. Relatively lower amylase (41.1 U/mL) and protease (14.2 U/mL) activities were attained in non-sterile medium. Graphic