Carbonic anhydrase from Camelia sinensis (tea) leaves

DEMIR Y., DEMIR N., Agar G.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, cilt.27, sa.4, ss.271-278, 1997 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 4
  • Basım Tarihi: 1997
  • Doi Numarası: 10.1080/10826069708001284
  • Dergi Adı: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.271-278
  • Atatürk Üniversitesi Adresli: Evet

Özet

Carbonic anhydrase (CA) (carbonate hydrolyase; E,C,4.2.1.1) from leaves of mature Camelia sinensis was purified and characterized. The purification level was 53 fold. The optimum temperature for maximal enzyme activity is 50 degrees C. The optimum pH was 6.8 and this pH varied between 6.5 and 7.5. Each enzyme molecule is a hexamer with an Mr of 169,000 with subunits of Mr=28,000.