A novel polyphenol oxidase immobilized polyglycine/reduced graphene oxide composite electrode for sensitive determination of catechol

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Dağcı Kıranşan K., Aksoy M.

JOURNAL OF APPLIED ELECTROCHEMISTRY, cilt.50, sa.8, ss.863-873, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 8
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s10800-020-01441-0
  • Dergi Adı: JOURNAL OF APPLIED ELECTROCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, Aquatic Science & Fisheries Abstracts (ASFA), Chemical Abstracts Core, Communication Abstracts, Compendex, Computer & Applied Sciences, INSPEC, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.863-873
  • Anahtar Kelimeler: Flexible paper, Polyphenol oxidase, Catechol detection, ELECTROCHEMICAL DETERMINATION, PAPER ELECTRODE, HYDROQUINONE, CARBON, RESORCINOL, SENSOR
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, we report a new type of flexible and free standing paper electrochemical sensor fabricated by integrating reduced graphene oxide (rGO), poly(glycine) and polyphenol oxidase (PPO) enzyme. For the sensor development, firstly, electrochemical polymerization of glycine at the rGO paper electrode was carried out by the cyclic voltammetric method, and then the poly(glycine)/rGO paper electrode was further utilized as a matrix to immobilize the PPO enzyme purified from potato. The developed PPO-poly(glycine)/rGO immobilized paper sensor was characterized by scanning electron microscopy, X-ray photoelectron spectroscopy and Raman spectroscopy. Enzyme aggregation and distribution within the poly(glycine)/rGO paper electrode were studied using a fluorescence microscope. The electrochemical behavior of catechole (CC) on PPO-poly(glycine)/rGO immobilized paper electrode was investigated by cyclic voltammetry and amperometry. The sensor performed well in the concentration range of 0.1-800 mu M, with a detection limit of 0.07 mu M and a sensitivity of 0.0512 mA mu M cm(-2).