Akademik Veri Yönetim Sistemi
Deutsche Tierarztliche Wochenschrift, cilt.111, sa.4, ss.162-165, 2004 (SCI-Expanded)
This study investigated the disposition kinetics and plasma availability of erythromycin in broiler chickens after single intravenous (iv), intramuscular (im), subcutaneous (sc) and oral administrations (po) of 30 mg kg-1 b. wt. Tissue residue profiles were also studied after multiple intramuscular, subcutaneous, and oral administration of 30 mg kg-1 b. wt., twice daily for three consecutive days. Plasma and tissue concentrations of erythromycin were determined using microbiological assay methods with Micrococcus luteus as the test organism. Following intravenous injection, plasma concentration-vs-time curves were best described by a two compartment open model. The decline in plasma drug concentration was bi-exponential with half-lives of (t1/2α,) 0.19 h and (t1/2β) 5.3 h for distribution and elimination phases, respectively. After intramuscular, subcutaneous and oral administration erythromycin at the same dose was detected in plasma at 10 min and reached its minimum level 8 h post-administration. The peak plasma concentration (Cmax) were 5.0, 5.3, and 6.9 μg·ml-1 and were attained at 1.7, 1.4, and 1.3 h (T max), respectively. The elimination half-lives (T1/2el) were 3.9, 2.6, and 4.1 h and the mean residence times (MRT) were 3.5, 3.2, and 3.6 h, respectively. The systemic bioavailabilities were 92.5, 68.8, and 109.3 %, respectively. In vitro protein binding percent of erythromycin in broiler plasma was ranged from 21 to 31 %. The limit of quantification (LOQ) for the assay was 0.03 μg·ml-1 in plasma and tissues. The tissue level concentrations were highest in the liver, and decreased in the following order: plasma > kidney > lung > muscle and heart. No erythromycin residues were detected in tissues and plasma after 24 h except in liver and kidney where it persisted during 48 h following intramuscular and oral administrations.