ICNAS 2021 (1st INTERNATIONAL CONGRESS ON NATURAL SCIENCES 2021, Erzurum, Türkiye, 10 - 12 Eylül 2021, ss.50-51
In spite of great
developments in the agricultural field and plant productivity in the last
decades, the concern about the control of agricultural pests is still
continuous. However, pest management is expected to have more effects from
nanomaterials by providing innovative solutions. The current study was provided
the biosynthesis of silver nanoparticles (AgNPs) using a cell-free culture
filtrate of metal silver-resistant bacteria Pseudomonas aeruginosa OG1. For
AgNPs production, Pseudomonas aeruginosa
OG1 strain was incubated for 24 h at 150 rpm and 30 ℃ in Nutrienth Broth. After
the incubation period, the solution was cleaned, separating the AgNPs from
unwanted contaminants and bacteria cells. The formed AgNPs were characterized using initially
visual observation of the change in the color of the reaction mixture from colorless
to the black as an indication of AgNP biotransformation. Biosynthesized
AgNPs was characterized using UV–Vis, XRD and SEM analysis. UV–Vis spectroscopy showed a maximum absorption at 430
nm under the optimum conditions performed. Aphis
pomi collected from apple leaves. The insectidial activity was studied
using leaf dip method. Leafs of host plant were dipped in different
concentrations of AgNPs solution (1000 ppm) and distilled water (control). The
activity was recorded after 24 h. The result suggests that AgNPs could be used
not only as a biocontrol agent, but also as an ecofriendly and inexpensive
approach for controlling the fruit pests.