Impact of long term Fe<SUP>3+</SUP> toxicity on expression of glutathione system in rat liver

BUDAK H., GONUL N., Ceylan H., KOCPINAR E.

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, sa.1, ss.365-370, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.etap.2013.12.007
  • Dergi Adı: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.365-370
  • Anahtar Kelimeler: Iron, Toxic effects, Glutathione system, mRNA expression, Enzyme activity, INDUCED OXIDATIVE STRESS, IRON-DEFICIENCY ANEMIA, CANCER-RISK, MAMMARY-GLAND, S-TRANSFERASE, DIETARY IRON, OVERLOAD, LEAD, METABOLISM, MECHANISMS
  • Atatürk Üniversitesi Adresli: Evet

Özet

The free radicals within the body, produced by metabolic activities or derived from environmental sources are relatively related to hepatoxicity. Since heavy metals including iron have the ability to produce free radicals, the liver glutathione system neutralizes them to protect cells against any damage. The objective of this study is to indicate the toxic effects of iron on the glutathione system at the enzymatic and molecular level. Thus, any possible correlation between enzymatic and molecular levels can be determined. According to our results, while mRNA expression of glutathione reductase (Gsr) and glutathione S-transferases (Gsta5) genes were not affected by long-term exposure to various concentrations of iron (Fe3+), transcription level of glutathione peroxidase (Gpx2) was influenced in the presence of toxic iron. Whereas the enzyme activites of GSR (GR), GPX and GST were significantly affected in rat liver. (C) 2013 Elsevier B.V. All rights reserved.