Metabolomics approach: Interpretation of changes in rat plasma metabolites after solifenacin treatment

Creative Commons License

AKMAN T. Ç., KADIOĞLU Y., ŞENOL O., ERKAYMAN B.

Brazilian Journal of Pharmaceutical Sciences, cilt.58, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1590/s2175-97902022e20849
  • Dergi Adı: Brazilian Journal of Pharmaceutical Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: Solifenacin succinate, Metabolomics, Rat, LC-Q-TOF, MS, MS, ANTIMUSCARINIC AGENT SOLIFENACIN, SMOOTH-MUSCLE-CELLS, OVERACTIVE BLADDER, DOUBLE-BLIND, CONTROLLED TRIAL, PROLIFERATION, RECEPTORS, EFFICACY, SAFETY
  • Atatürk Üniversitesi Adresli: Evet

Özet

© 2022, Faculdade de Ciencias Farmaceuticas (Biblioteca). All rights reserved.Changes in metabolite levels of patients using the long-term drug can be comprehensively demonstrated by pharmacometabolomic studies. In this study, biological alterations induced by the administration of solifenacin succinate were investigated with a pharmacometabolomics approach on rat metabolism. Plasma samples obtained from rats were analyzed by LC-Q-TOF/MS/MS. METLIN and HMDB databases were used to identify metabolites. Data were processed and classified with MATLAB 2017b. 53 m/z values were found to be significantly different between the drug and control groups (p ≤ 0.01 and fold analysis > 1.5) and identified by comparing METLIN and HMDB databases. According to multivariate data analysis, changes in arachidonic acid, thromboxane A2, palmitic acid, choline, calcitriol, histamine phosphate, retinyl ester, l-cysteine, l-leucine, beta-alanine, l-histidine levels were found to be statistically significant compare to the control group. Differences in the biosynthesis of phenylalanine, aminoacyl-tRNA, tyrosine, tryptophan, metabolism of glycerophospholipid, cysteine, methionine, histidine, arachidonic metabolism have been successfully demonstrated by the metabolomics approach. Our study provides important information to explain the efficacy and toxicity of chronic administration of solifenacin succinate.