Detoxification and reproductive system-related gene expression following exposure to Cu(OH)(2) nanopesticide in water flea (Daphnia magna Straus 1820)

Aksakal F., Arslan H.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, cilt.27, sa.6, ss.6103-6111, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 6
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s11356-019-07414-x
  • Dergi Adı: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, IBZ Online, ABI/INFORM, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.6103-6111
  • Atatürk Üniversitesi Adresli: Evet

Özet

The extensive use of copper-based nanopesticides in agriculture has led to their release into the aquatic environment and causes a potential risk to aquatic biota. However, there is a lack of knowledge regarding the possible toxic effect of these nanopesticides on non-target aquatic organisms including invertebrates. Therefore, in this study, effects of commonly used copper-based nanopesticide "Kocide 3000" on gene expression related to detoxification (cyp360a8, gst, P-gp, and hr96) and reproductive system (cut, cyp314, dmrt93, and vtg) in Daphnia magna was investigated through an acute toxicity test. In general, exposure to the nanopesticide caused significant down-regulation of detoxification genes after 24 h and then significant up-regulation after 48 h. Exposure to the nanopesticide, however, significantly induced cut expression after 24 h. Moreover, dmrt93 and vtg genes were up-regulated after 48 h exposure to the nanopesticide. On the other hand, the expression of dmrt93 and vtg down-regulated at high concentration of Cu(OH)(2) nanopesticide (1.5 ppm) after 96 h. The results of this study provide first evidence into the crucial role of genes related to detoxification and reproductive system in response to Cu(OH)(2) nanopesticide. The use of physiological, biochemical bioassays, as well as gene expression, can help explain the toxic effect of copper- based nanopesticides and provide more insight into the exact mechanism of toxicity in non-target aquatic organisms.