ADVANCES IN NANO RESEARCH, cilt.17, sa.1, ss.75-86, 2024 (SCI-Expanded)
The insecticidal efficiency of orange (Citrus sinensis) peel essential oil (OP-EO) is limited because of its low
stability under environmental conditions. Nanoemulsion formulations show promise in overcoming this limitation. Therefore,
this study aimed to formulate and characterize the OP-EO nanoemulsion form (OP-EON) and investigate its insecticidal
properties against two significant storage pests, Rhyzopertha dominica (Fabricius, 1792), and Tribolium castaneum (Herbst,
1797). The OP-EON (4:3:3:90 w/w, EO: Tween 80: Ethanol: water) was successfully created using an ultrasonic homogenizer.
The major chemical components of the OP-EO were determined to be D-limonene (87.93%), myrcene (3.62%), and α-pinene
(1.34%) through GC-MS analysis. The OP-EON was characterized using TEM (50-100 nm), Zeta sizer (the mean droplet
particle size of 58.60 nm, the ζ-potential value of -12.6 mV, and the polydispersity index of 0.486), and FT-IR analysis. After 7
days, exposure to 500 ppm of the OP-EON resulted in 50% and 30% mortality rates in R. dominica and T. castaneum,
respectively. Exposure to 1000 ppm of OP-EON resulted in 90% and 55% mortality in R. dominica and T. castaneum,
respectively, after 7 days. Overall, these results clearly showed the potential to exceed the limits of the insecticidal activity of the
OP-EO with its nanoemulsion form