Effect of taxifolin on development of retinopathy in alloxan-induced diabetic rats


Ahiskali I., Pinar C. L., Kiki M., MAMMADOV R., ÖZBEK BİLGİN A., HACIMÜFTÜOĞLU A., ...Daha Fazla

CUTANEOUS AND OCULAR TOXICOLOGY, cilt.38, sa.3, ss.227-232, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 3
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1080/15569527.2019.1588289
  • Dergi Adı: CUTANEOUS AND OCULAR TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.227-232
  • Anahtar Kelimeler: Alloxan, diabetic retinopathy, retina toxicity, taxifolin, rat, OXIDATIVE STRESS, ADVANCED GLYCATION, ANTIOXIDANTS, PRODUCTS, PATHOGENESIS
  • Atatürk Üniversitesi Adresli: Evet

Özet

Purpose: Diabetic retinopathy (DR) is one of the leading causes of blindness. In DR patients, antioxidant defence is disrupted, and production of reactive oxygen species and pro-inflammatory cytokines such as interleukin 1 beta (IL-1 beta) and tumour necrosis factor alpha (TNF-alpha) increases. Taxifolin has been reported to suppress reactive oxygen species, IL-1 beta and TNF-alpha production. The aim of this study is to biochemically and histopathologically examine the protective effect of taxifolin against DR damage induced by alloxan. Materials and methods: Alloxan received rats with a blood glucose level of >= 250 mg/dL were divided into taxifolin-treated (TAX) (n = 6), diabetic control (DC) (n = 6) groups. There were rats received only saline in non-diabetic control (NC) group (n = 6). Taxifolin (50 mg/kg) was orally administered to the TAX group rats. DC and NC rats received the same volume of saline as a solvent. This procedure was repeated once a day for 3 months. At the end of this period, animals were killed by high dose thiopental sodium anaesthesia. Histopathological examinations were then performed on excised rat eyes. Malondialdehyde (MDA), total glutathione (tGSH), IL-1 beta and TNF-alpha levels were measured in obtained blood samples. Results: MDA, IL-1 beta and TNF-alpha levels were significantly increased in blood samples of DC group rats with hyperglycemia induced by alloxan compared with NC group (p < 0.0001), and decreased in the TAX group compared with the DC group (p < 0.0001). The levels of tGSH were significantly decreased in blood samples of DC group rats compared with NC group (p < 0.0001), and increased in the TAX group compared with the DC group (p < 0.0001). Histopathologically, retinal ganglion cells of the TAX group had a slightly dilated and congested blood vessel, and severe damage was inflicted to the retinal ganglion cell layer of the DC group. Conclusions: Experimental results suggest that taxifolin may be beneficial in the treatment of DR.