Purification and Properties of Peroxidase from Prangos ferulacea (Apiaceae) and Investigation of Inhibition by Some Chemicals

Nadaroglu H.

ASIAN JOURNAL OF CHEMISTRY, cilt.21, sa.7, ss.5768-5776, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 7
  • Basım Tarihi: 2009
  • Dergi Adı: ASIAN JOURNAL OF CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.5768-5776
  • Atatürk Üniversitesi Adresli: Evet

Özet

A peroxidase (POD)-containing fraction was purified from Prangos ferulacea (apiaceae) by (NH4)(2)SO4 precipitation and anion exchange chromatography. The purification grade obtained was 126.36 yielding 41.8% of the enzyme activity. The molecular weight was estimated to be 40 kDa by gel filtration. It has an optimum pH of 5 and exhibited high pH and thermal stabilities. Km for guaiacol and ABST were 1.347 and 1 mM, respectively. The optimal temperature was 30 degrees C. The 150 values for KSCN and NaN3 were 0.750 mM, 1.321 mM in Prangos ferulacea (Apiaceae) CA, respectively.