Molecular identification of Bacillus pumilus isolated from natural resources by 16S rRNA, chitinase enzyme activity and the industrial relevance of the related enzyme


Dikbaş N., Kızıler Y. E., Cinisli K. T., Uçar S., Canca E.

ECOMB 2019: Eurasian Congress on Molecular Biotechnology, Trabzon, Türkiye, 19 - 21 Eylül 2019, Trabzon, Türkiye, 19 - 21 Eylül 2019, ss.20-21

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: Trabzon
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.20-21
  • Atatürk Üniversitesi Adresli: Evet

Özet

The chitinase which is identified in the literature is also known with is chemical name β-1,4-N- acetyl glucosamine. Chitinases are produced by plants, animals and microorganisms. Microbial-derived chitinases are preferred for biotechnological applications. Chitinases are preferred in food industry, feed additives, paper industry and soil improvement. In biotechnology, chitinase enzyme studies are an effective tool to be used in the production of enzymes, or in the development of their properties.

The aim of this study was to investigate the molecular identification of Bacillus pumilus strain isolated from fatty cheese by 16S rRNA, to determine its chitinase enzyme activity and to evaluate its possible industrial effectiveness. The identification was performed using the 16S rRNA method. The identification rate of Bacillus pumilus was 99 % and the optimum temperature of the extracellular chitinase enzyme was determined as 40°C and the optimum pH was pH 7.0. According to the results, it has been confirmed with our study that the bacterium can be introduced to the industrial use due to the characteristics of the enzyme produced by this bacterium and that it can be used as a starter culture in the food industry.