Introduction and Purpose: The aim of this study is to investigate the isokinetic knee strength and balance performance of professional football players by position. Materials and Methods: The study group of the research consisted of professional football players from Erzurumspor F.K. who will compete in the T.F.F. Trendyol 1st League during the 2023-2024 season. A total of 26 players participated in the study, with 13 defenders and 13 attackers. To determine the players' body compositions, the BODPOD Gold Standard tracking system was used. Static and dynamic balance tests were conducted using the SPORTKAT 4000 device, and the ISOMED 2000 Isokinetic system was used to determine knee flexion-extension forces. After testing the normality distribution of the measured parameters using Skewness, Kurtosis, and Shapiro-Wilks tests, it was determined that the data showed a normal distribution. Independent Samples t-tests were applied to identify differences between positions. The significance level for this study was set at p<0.05. Findings: According to the results obtained from the research, it was found that there was a significant difference in hamstring strength values in favor of the attacking players in both legs, and in the quadriceps strength values in favor of the attacking players in the left leg. Statistically significant differences were also identified in static balance values in favor of defenders and in dynamic balance values in favor of attackers. Results: In conclusion, our study revealed differences between football players in different positions, likely due to the varying physical and physiological requirements of their respective roles.