This study was aimed to investigate the possible effects of acute training on hypofyse hormone levels and some biochemical analytes in kick-boxers. The study was included 23 healthy professional male kick-boxers (age range: from 17 to 32 years and BMI range: from 19.0 to 24.2). Sportsmen were exposed to a total of 95-minute training program including training and a kick-boxing match. Venous blood samples and urine were taken from sportsmen just before and immediately after training and some biochemical analytes were analyzed. The evaluation of pre- and post-training serum levels of analytes have shown that growth hormone, adrenocorticotropic hormone, prolactine, throide stimulating hormone, adrenalin and noradrenalin levels were significantly increased and serum insulin-like growth factor I and free thyroxine levels were significantly decreased by training. There were no significant differences between pre- and post-training serum levels of other biochemical analytes; luteinizing hormone, follicle-stimulating hormone, free triiodothyronine, cortisole and dopamin. Our results have revealed the fact that exercise alters pituitary functions and this increment is associated with response to exercise stress. "We are in agree with the previous recommendations about regulary screening of pituitary functions and total hormonal status of competing and retired kick-boxers".