Akademik Veri Yönetim Sistemi
9th International Baskent Congress on Life, Engineering, and Applied Sciences, Ankara, Türkiye, 20 - 23 Mayıs 2023, ss.160-165
Aeromonas caviae, belongs to the family Aeromonadaceae is a Gram-negative opportunistic pathogen
causing disease in fish under stressful conditions. Because of its high mortality rates and deterioration
of product quality, the bacterium causes significant economic losses in fish farms. Intensive use of
antibiotics both to support fish growth and against bacterial diseases has resulted in the development
of drug-resistant bacterial strains. Due to their toxicity and the side effects mentioned, researchers
focused on finding new alternatives to enhance fish immunity and treat bacterial infections. Recently,
popularity and scientific interest in essential oils derived from aromatic herbs, which are thought to be
rich sources of antibacterial capabilities, have increased. Laurus nobilis, commonly known as laurel, bay
laurel or daphne which belongs to Lauraceae family is an evergreen tree that grows wild or cultivated.
Recent studies emphasize the antimicrobial, antifungal, anti-inflammatory, anticonvulsant, antioxidant,
antidiabetic, anticancer, anticholinergic and neuroprotective activities of L. nobilis leaf extracts and
essential oils.
The present study was intended to evaluate the invitro antimicrobial susceptibilities of Laurel (Laurus
nobilis L.) essential oil against Fish Pathogen Aeromonas cavie. Isolation and identification of the main
components of essential oil identified as 1,8-cineole and α-terpinyl acetate. The McFarland 0.5 standard
was used to determine the study inoculums, and the standard disk diffusion technique was used to analyze
the antimicrobial activity. Erythromycin (E15 μg), Netilmicin (N30 μg), Chloramphenicol (C30 μg),
and Amoxycillin (A10 μg) were used as positive controls. The essential oils from L. nobilis possessed
antibacterial activity against tested A. caviae at 1-10 μg/disc. The antibacterial effect was determined to
be concentration dependent. Our findings showed that L. nobilis essential oil made an excellent source
of anti-A. caviae. Therefore, using L. nobilis essential oil in the fish processing operations may have
potential as a natural antibacterial agent against food-borne pathogens and spoilage microorganisms.