Comparison of the efficacy of the mouse hepatic and renal antioxidant systems against inflammation-induced oxidative stress

Hukkamlı B., Dağdelen B., Sönmez Aydın F., BUDAK H.

Cell Biochemistry and Biophysics, cilt.81, sa.2, ss.299-311, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 81 Sayı: 2
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s12013-023-01126-3
  • Dergi Adı: Cell Biochemistry and Biophysics
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.299-311
  • Anahtar Kelimeler: Inflammation, Oxidative stress, Thioredoxin system, Glutathione system, Expression and activity, THIOREDOXIN-INTERACTING-PROTEIN, EARLY EMBRYONIC LETHALITY, GENE-EXPRESSION, CYTOSOLIC THIOREDOXIN, REDOX STATE, REDUCTASE, APOPTOSIS, TISSUE, OVEREXPRESSION, DISEASE
  • Atatürk Üniversitesi Adresli: Evet

Özet

© 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.This study was conducted to compare the efficacy of the mouse hepatic and renal antioxidant systems against inflammation-induced oxidative stress. Increased Il-1 and Il-6 expressions, markers of inflammation, were represented by inflammation models in mouse liver and kidney tissues injected intraperitoneally with LPS. After establishing the model, the GSH level and the GSH/GSSG ratio, which are oxidative stress markers, were investigated in both tissues treated with LPS and the control group. The expression of Trx1, TrxR, and Txnip genes increased in the liver tissues of LPS-treated mice. In the kidney tissue, while Trx1 expression decreased, no change was observed in TrxR1 expression, and Txnip expression increased. In the kidneys, TRXR1 and GR activities decreased, whereas GPx activity increased. In both tissues, the TRXR1 protein expression decreased significantly, while TXNIP expression increased. In conclusion, different behaviors of antioxidant system members were observed during acute inflammation in both tissues. Additionally, it can be said that the kidney tissue is more sensitive and takes earlier measures than the liver tissue against cellular damage caused by inflammation and inflammation-induced oxidative stress.