Akademik Veri Yönetim Sistemi
TOXICOLOGY AND INDUSTRIAL HEALTH, cilt.31, sa.1, ss.44-51, 2015 (SCI-Expanded)
In this study, potential genotoxic effects of ethyl methanesulfonate (EMS) that caused mutagenicity in a variety of organisms were tried to resolve by the methanol and chloroform extract of Echium amoenum (EA(met) and EA(chl)) Fisch. & C.A. Mey. from the family of Boraginaceae, which is an endemic plant, and is used as an alternative treatment among public in Iran. Somatic mutation and recombination test with Drosophila wing was used to determine the genotoxic and antigenotoxic effects in our investigations. For this purpose, 3-day-old transheterozygous larvae of mwh/flr(3) genotype of Drosophila melanogaster were used in all our experiments. The larvae were fed chronically on the Drosophila instant medium (DIM) including 1ppm EMS. However, in another application group, different concentrations (1, 2 and 4ppm) of EA(met) and EA(chl) were added to DIM including 1ppm EMS (EMS+EA(met) and EMS+EA(chl)). Then, for the matured individuals, wing preparates were prepared within the mediums that include control group that has only DIM, negative control group that contains dimethyl sulfoxide and application groups in different concentrations that contain EMS, EMS+EA(met) and EMS+EA(chl). Clone induction frequency for the normal wing phenotype of EMS application group was observed to be 2.00. In the EMS+EA(met) application group, the value of 1ppm EA(met) is 1.49, value of 2ppm EA(met) is 1.08 and value of 4ppm EA(met) is 0.72; in the EMS+EA(chl) application group, the value of 1ppm is EA(chl) 1.33, value of 2ppm EA(chl) is 0.67 and value of 4ppm EA(chl) is 0.56 were determined. This decrease observed between EMS and all application groups in terms of total induction frequency is statistically significant (p<0.05). These results concluded that chloroform extracts were more effective than the methanol extracts of E. amoenum.