Antidiabetic and antioxidant properties of Paeonia mascula L.: In vitro and in vivo studies, and phytochemical analysis

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Gözcü S., Uğan R. A., Özbek H., Gündoğdu B., Güvenalp Z.

Fitoterapia, cilt.170, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 170
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.fitote.2023.105658
  • Dergi Adı: Fitoterapia
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, CINAHL, EMBASE, International Pharmaceutical Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Antidiabetic, Antioxidant, Paeonia mascula L., α-Glucosidase
  • Atatürk Üniversitesi Adresli: Evet

Özet

The medicinal plant Paeonia mascula L. is commonly used in Anatolian folk medicine for its antidiabetic properties. This study aimed to investigate the in vitro α-glucosidase enzyme inhibition effect, in vivo antidiabetic, and antioxidant activities of extracts obtained from P. mascula. The in vivo studies were conducted on diabetic rats induced with streptozotocin. The ethyl acetate and n-butanol extracts showed the highest efficacy in both in vitro and in vivo experiments, reducing AST, ALT, and MDA levels while increasing GSH and SOD activities in rats. In total, seven compounds were isolated from both extracts, and their structures were identified using spectroscopic methods such as 1D and 2D NMR and Mass Spectrometry. The in vitro α-glucosidase inhibition assay on purified compounds revealed that 1,2,3,4,6-penta-O-galloyl-β-D-glucose was the most effective compound. These findings support the traditional use of P. mascula as an antidiabetic agent.