From Computationally Guided Hapten Design to Monoclonal Antibody Production: A Novel Strategy for Rapid and Sensitive Detection of Flusilazole Residues
Analytical Chemistry, cilt.98, sa.26, ss.19776-19786, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 98 Sayı: 26
- Basım Tarihi: 2026
- Doi Numarası: 10.1021/acs.analchem.6c02775
- Dergi Adı: Analytical Chemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, Chimica, Compendex, EMBASE, MEDLINE, Natural Science Collection (ProQuest), Earth, Atmospheric, & Aquatic Science Collection (ProQuest), Materials Science & Engineering Collection (ProQuest), Pharma Collection (ProQuest), Technology Collection (ProQuest)
- Sayfa Sayıları: ss.19776-19786
- Atatürk Üniversitesi Adresli: Evet
Özet
Flusilazole, a widely used triazole fungicide, poses potential risks through its residues in food and the environment. However, rapid surveillance of flusilazole residue remains limited by the lack of reported high-performance antibodies. Herein, we report a high-performance monoclonal antibody (mAb) against flusilazole using a computationally guided hapten design strategy. Among the four candidate haptens evaluated through molecular simulation of three-dimensional conformation, electrostatic potential mapping, and electronic structure descriptors, Hapten 01 exhibited the highest structural and electrostatic similarity to flusilazole. This hapten yielded a high-sensitivity monoclonal antibody (mAb-6H9) with excellent sensitivity (IC50 value of 1.99 μg/L) and specificity (cross-reactivity rate <0.01%). On this basis, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold test strip (CGTS) were developed for rapid residue analysis, and the detection limits of the two assays were 0.46 and 1.18 μg/L in buffer, respectively. In spiked cucumber, strawberry, and orange samples, satisfactory recoveries (71–108%) and good precision were achieved. This study highlights the effectiveness of computationally guided hapten design and establishes a monoclonal antibody-enabled rapid immunoassay dual-platform format for flusilazole detection.