AKRİLAMİD NEDENLİ PERİFERİK KAN VE KEMİK İLİĞİ TOKSİSİTESİNDE KARVAKROL'UN MUHTEMEL KORUYUCU ETKİLERİNİN ARAŞTIRILMASI

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Atatürk Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler, Türkiye

Tezin Onay Tarihi: 2023

Tezin Dili: Türkçe

Öğrenci: ILHAM MAMMADOV

Danışman: Fatma Gür

Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu

Özet:

Aim: Acrylamide (AC) is a compound formed in foods processed at high temperatures and has carcinogenic effects. Considering such health risks, studies to reduce or prevent AC exposure and toxicity are of great importance. In this context, it was aimed to evaluate the potential protective effects of carvacrol (KAR), a monoterpenoid phenol compound found in plant sources, against AC-induced toxicity. Material and method: A total of 30 Sprague Dawley rats were used for this study and these rats were divided into 5 different groups (n=6 in each group). The control group was given saline. KAR group received 100 mg/kg intraperitoneal carvacrol for 21 days. AC group received 25 mg/kg oral injections. The remaining two groups received low and high-dose KAR and AC combinations. At the end of the experiment, peripheral blood and bone marrow were collected from the rats and analyzed. Results: In this study, possible protective effects of KAR against Hb and Hct toxicity of AC in addition to the haematopoietic system were determined. Biochemical analyses and statistical evaluations of bone marrow and peripheral blood tissues from rats showed AC-induced toxic effects on erythrocytes, platelets, haemoglobin, haematocrit and bone marrow values. When the protective effects of KAR on AC-induced toxic effects were evaluated, it was found that AC toxicity was prevented in haematological parameters and the values were close to the control, but both doses of KAR administration could not prevent bone marrow toxicity. Conclusion: In vivo exposure to AC has been shown to cause toxicity in rat bone marrow and hematological parameters. As AC is a reactive organic compound, it plays an active role in the nucleophilic attack of amino acids, peptides, and proteins, and excessive production of reactive oxygen species leading to AC oxidative stress was explained as the underlying mechanism of AC-induced haematotoxicity. Further studies are needed to prevent bone marrow toxicity of AC. Key Words: Acrylamide, carvacrol, toxicity, antioxidant, in vitro, in vivo, rat