Recognition elements based on the molecular biological techniques for detecting pesticides in food: A review.

Zhao M., Wang M., Zhang X., Zhu Y., Cao J., She Y., ...Daha Fazla

Critical reviews in food science and nutrition, cilt.63, sa.21, ss.4942-4965, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 63 Sayı: 21
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/10408398.2021.2009762
  • Dergi Adı: Critical reviews in food science and nutrition
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, CINAHL, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, Metadex, SportDiscus, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.4942-4965
  • Anahtar Kelimeler: Molecular biology techniques, recognition elements, biosensor, immunoassay technology, APTAMER-BASED SENSOR, SENSITIVE DETECTION, CELL-SURFACE, ELECTROCHEMICAL APTASENSOR, LABEL-FREE, ORGANOPHOSPHORUS PESTICIDES, PICHIA-PASTORIS, ACETYLCHOLINESTERASE, EXPRESSION, ANTIBODIES
  • Atatürk Üniversitesi Adresli: Evet

Özet

Excessive use of pesticides can cause contamination of the environment and agricultural products that are directly threatening human life and health. Therefore, in the process of food safety supervision, it is crucial to conduct sensitive and rapid detection of pesticide residues. The recognition element is the vital component of sensors and methods for fast testing pesticide residues in food. Improper recognition elements may lead to defects of testing methods, such as poor stability, low sensitivity, high economic costs, and waste of time. We can use the molecular biological technique to address these challenges as a good strategy for recognition element production and modification. Herein, we review the molecular biological methods of five specific recognition elements, including aptamers, genetic engineering antibodies, DNAzymes, genetically engineered enzymes, and whole-cell-based biosensors. In addition, the application of these identification elements combined with biosensor and immunoassay methods in actual detection was also discussed. The purpose of this review was to provide a valuable reference for further development of rapid detection methods for pesticide residues.