Preparation of chitosan from waste shrimp shells fermented with Paenibacillus jamilae BAT1

Taser B., Ozkan H., Adiguzel A., Orak T., Baltaci M. Ö., Taskin M.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.183, ss.1191-1199, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 183
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.ijbiomac.2021.05.062
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1191-1199
  • Anahtar Kelimeler: Chitin extraction, Deproteinization, Demineralization, chitosan, Antibacterial, Antioxidant, LOW-MOLECULAR-WEIGHT, CHITIN EXTRACTION, ANTIBACTERIAL ACTIVITY, PROTEIN HYDROLYSATE, BACILLUS-LICHENIFORMIS, ALKALINE PROTEASE, N-DEACETYLATION, GROWTH, YIELD, OPTIMIZATION
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, chitin extraction from shrimp shell powder (SSP) using locally isolated Paenibacillus jamilae BAT1 (GenBank: MN176658), the preparation of chitosan from the extracted chitin, and the characterization and biological activity (antimicrobial and antioxidant) of the prepared chitosan (PC) were investigated. It was determined that P. jamilae BAT1 did not have chitinase activity but showed high protease activity and protein removal potential. Optimum pH, shell concentration and incubation time for deproteinization were determined as 7.0, 60 g/L and 4 days, respectively. Addition of KH2PO4 or MgSO4 did not affect chitin extraction and deproteinization yield. The maximum yields of deproteinization, demineralization and chitin extraction yields were 87.67, 41.95 and 24.5%, respectively. The viscosity-average molecular weight of PC was determined as 1.41 x 10(5) g/mol. The deacetylation degree of PC (86%) was found to be higher that of commercial chitosan (CC) (78%). DPPH center dot scavenging activity of PC (IC50 0.59 mg/mL) was higher than that of CC (IC50 3.72 mg/mL). PC was found to have higher antimicrobial activity against the bacteria E. coli and S. aureus and the yeast C. albicans when compared to CC. This is the first study on the use of the bacterium P. jamilae in biological chitin extraction . (C) 2021 Published by Elsevier B.V.