Dual-mode detection of organophosphate pesticides in pear and Chinese cabbage based on fluorescence and AuNPs colorimetric assays

Yu H., Wang M., Cao J., She Y., Zhu Y., Ye J., ...Daha Fazla

Food Chemistry, cilt.364, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 364
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.foodchem.2021.130326
  • Dergi Adı: Food Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Fluorescence, Colorimetric, Dual-mode detection, Organophosphate pesticides, Residue detection, Enzyme inhibition, GOLD NANOPARTICLES, ACETYLCHOLINESTERASE, DICHLORVOS
  • Atatürk Üniversitesi Adresli: Evet

Özet

© 2021 Elsevier LtdHerein, a dual-mode method based on fluorescent and colorimetric sensor was developed for determination of organophosphate pesticides (OPs). In this study, indoxyl acetate (IDA) was hydrolyzed by esterase into indophenol. Indophenol leads to changes in fluorescence signal and aggregation of gold nanoparticles (AuNPs); ultimately changing the color from red to blue. When OPs exist, the formation of indophenol was inhibited. With increasing the concentrations of OPs, the enhancement rate of fluorescence signal decreases, and the color change of AuNPs weakened gradually. The assay was applied for determination of dichlorvos, trichlorfon, and paraoxon, and the limits of detection (LODs) were 0.0032 mg/kg, 0.0096 mg/kg, and 0.0074 mg/kg (fluorometric assay), and 0.0120 mg/kg, 0.0224 mg/kg, and 0.0106 mg/kg (colorimetric assay), respectively. Finally, such a convenient and sensitive sensing assay was successfully applied for quantification of OPs in pear and Chinese cabbage with good recoveries ranged between 80.19 and 116.93%.