1st International Agricultural Structures and Irrigation Congress, Antalya, Türkiye, 26 - 28 Eylül 2018, ss.45
Plants require several abiotic conditions during their vegetation periods. In case of unfavorable abiotic conditions stress occurs and this can be tolerated by internal production of antioxidant enzymes. In this study, the change in enzyme level of carrot plants that were exposed to 6 different irrigation water salinity (To =0,38 dS/m, T1 =1,5 dS/m, T2 =3 dS/m, T3 =5 dS/m, T4 =7 dS/m, T5 =10 dS/m) in 6 days intervals of irrigation, was investigated.
Leaf samples were collected on the harvest period from carrots which were cultivated under salt stress, and important stress indicator enzymes (glutathione reductase (GR), glutathione s-transferase (GST), superoxide dismutase (SOD)) were calculated.
Due to increased irrigation water salinity; GST, GR
and SOD amounts of plants was observed to increase in parallel. Under increased
salinity of irrigation water, GST activity was increased till T4 subject, then
decreased in T5 subject. Activity of GR enzyme was shown to be similar. Based
on the stress caused by the increased salinity in all subjects, SOD activity
was increased until T5 subject and reached to the top level. As a
salt-sensitive plant, T0 enzyme activities of carrot plant were calculated to
be in the highest level.
As a result of the study; GST, GR and SOD enzymes
were proven to be an indicator of plant stress level, by showing activity
according to the amount of environmental drought, indicating that these
parameters can be utilized in planning of irrigation time and evaluation of
plant yield.